The bill was approved after developers announced they wanted to build the $150 million to $200 million Bible Park USA on 280 acres in the Blackman community to the west of Murfreesboro.
Gov. Phil Bredesen has signed the privilege tax into law.
The approval of the privilege tax on the local front requires that the 21-member County Commission pass a resolution by a two-thirds vote on two consecutive readings.
The legislation also allows for a referendum if 10 percent of qualified voters who voted in the last gubernatorial election file a petition with the county's Election Commission within 30 days of final approval of the resolution.
Rutherford County had 62,334 residents vote in the governor's election last November, meaning 6,233 of those voters must sign the petition to force a referendum on the issue.
"We would like to stop it before it gets that far," said Joe Dassaro, a Blackman area resident who has launched a Web site dedicated to preventing the theme park from being built.