Research suggests men are more likely to use humour aggressively by making others the butt of the joke.
And aggression - generally considered to be a more masculine trait - has been linked by some to testosterone exposure in the womb.
Professor Shuster believes humour develops from aggression caused by male hormones.
He documented the reaction of over 400 individuals to his unicycling antics through the streets of Newcastle upon Tyne.
Almost half of people responded verbally - more being men. Very few of the women made comic or snide remarks, while 75% of the men attempted comedy - mostly shouting out "Lost your wheel?", for example.